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*Main bibliograpy
1 [[Demonstration of Epstein-Barr Virus Localized in the Colonic and Ileal Mucosa of a Patient with Ulcerative Colitis and review of the literature.>http://www.ncbi.nlm.nih.gov/pubmed/10650270?dopt=Abstract]]
2 [[A case report of spontaneous regression of hepatocellular carcinoma and review of literature.>http://www.ncbi.nlm.nih.gov/pubmed/11059943?dopt=Abstract]]
3 [[Analysis of liver single photon emission computed tomography in a case of fulminant hepatic failure.>http://www.ncbi.nlm.nih.gov/pubmed/10587017?dopt=Abstract]]
4 [[Case report: Occurrence of hepatocellular carcinoma 4.5 years after successful treatment with virus clearance for chronic hepatitis C.>http://www.ncbi.nlm.nih.gov/pubmed/10535477?dopt=Abstract]]
5 [[Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge.>http://www.ncbi.nlm.nih.gov/pubmed/11227675?dopt=Abstract]]
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**Demonstration of Epstein-Barr Virus Localized in the Colonic and Ileal Mucosa of a Patient with Ulcerative Colitis and review of the literature
Authors: Yoshio Takeda, MD1, Kenzo Takada, MD2, Hitoshi Togashi, MD1, Hiroaki Takeda, MD1, Makoto Sakano, MD3, Yumiko Takeda, MD3, Yukie Osada, MD4, Haruhide Shinzawa, MD1 and Tsuneo Takahashi, MD1
1 The Second Department of Internal Medicine, Yamagata University School of Medicine,
Yamagata 990-9585, Japan.
2 Department of Virology, Cancer Institute, Hokkaido University School of Medicine, Japan.
3 Department of Internal Medicine, Shinoda General Hospital, Japan.
4 Department of Dermatology, Shinoda General Hospital, Japan.
Reprint requests: Yoshio Takeda, M.D. The Second Department of Internal Medicine, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata 990-9585, Japan
***Abstract
Background: There has been no report on Epstein-Barr virus localized in the human colonic mucosa.
Methods: In the rectal biopsy specimens from a patient with ulcerative colitis, detection of Epstein-Barr virus and human herpesvirus 6 was performed by the single strand PCR method. To localize Epstein-Barr virus in the paraffin-embedded biopsy specimens from hemorrhagic inflamed rectal mucosa and faintly inflamed terminal ileal mucosa, we attempted to detect Epstein-Barr virus-encoded small RNA-1 by in situ hybridization and Epstein-Barr virus latent membrane protein-1 by immunohistochemical staining.
Results: Epstein-Barr virus and human herpesvirus 6 were detected in the rectal biopsy specimens by the single strand PCR. Positive signals for Epstein-Barr virus-encoded small RNA-1 were recognized in nuclei of some epithelial cells from both the rectum and the terminal ileum. Positive staining for Epstein-Barr virus latent membrane protein-1 was detected in cytoplasm of some epithelial cells and mucosal lymphocytes of the rectal and the terminal ileal mucosa.
Conclusions: This report describes a patient with ulcerative colitis, the onset of which coincided with active Epstein-Barr virus and human herpesvirus 6 infection. This is the first demonstration of Epstein-Barr virus in the epithelial cells of the human colon by in situ hybridization and immunohistochemical staining.
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*Main bibliograpy
1 [[Demonstration of Epstein-Barr Virus Localized in the Colonic and Ileal Mucosa of a Patient with Ulcerative Colitis and review of the literature.>http://www.ncbi.nlm.nih.gov/pubmed/10650270?dopt=Abstract]]
2 [[A case report of spontaneous regression of hepatocellular carcinoma and review of literature.>http://www.ncbi.nlm.nih.gov/pubmed/11059943?dopt=Abstract]]
3 [[Analysis of liver single photon emission computed tomography in a case of fulminant hepatic failure.>http://www.ncbi.nlm.nih.gov/pubmed/10587017?dopt=Abstract]]
4 [[Case report: Occurrence of hepatocellular carcinoma 4.5 years after successful treatment with virus clearance for chronic hepatitis C.>http://www.ncbi.nlm.nih.gov/pubmed/10535477?dopt=Abstract]]
5 [[Growth inhibition and apoptosis of gastric cancer cell lines by Anemarrhena asphodeloides Bunge.>http://www.ncbi.nlm.nih.gov/pubmed/11227675?dopt=Abstract]]
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**Demonstration of Epstein-Barr Virus Localized in the Colonic and Ileal Mucosa of a Patient with Ulcerative Colitis and review of the literature
Authors: Yoshio Takeda, MD1, Kenzo Takada, MD2, Hitoshi Togashi, MD1, Hiroaki Takeda, MD1, Makoto Sakano, MD3, Yumiko Takeda, MD3, Yukie Osada, MD4, Haruhide Shinzawa, MD1 and Tsuneo Takahashi, MD1
1 The Second Department of Internal Medicine, Yamagata University School of Medicine,
Yamagata 990-9585, Japan.
2 Department of Virology, Cancer Institute, Hokkaido University School of Medicine, Japan.
3 Department of Internal Medicine, Shinoda General Hospital, Japan.
4 Department of Dermatology, Shinoda General Hospital, Japan.
Reprint requests: Yoshio Takeda, M.D. The Second Department of Internal Medicine, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata 990-9585, Japan
***Abstract
Background: There has been no report on Epstein-Barr virus localized in the human colonic mucosa.
Methods: In the rectal biopsy specimens from a patient with ulcerative colitis, detection of Epstein-Barr virus and human herpesvirus 6 was performed by the single strand PCR method. To localize Epstein-Barr virus in the paraffin-embedded biopsy specimens from hemorrhagic inflamed rectal mucosa and faintly inflamed terminal ileal mucosa, we attempted to detect Epstein-Barr virus-encoded small RNA-1 by in situ hybridization and Epstein-Barr virus latent membrane protein-1 by immunohistochemical staining.
Results: Epstein-Barr virus and human herpesvirus 6 were detected in the rectal biopsy specimens by the single strand PCR. Positive signals for Epstein-Barr virus-encoded small RNA-1 were recognized in nuclei of some epithelial cells from both the rectum and the terminal ileum. Positive staining for Epstein-Barr virus latent membrane protein-1 was detected in cytoplasm of some epithelial cells and mucosal lymphocytes of the rectal and the terminal ileal mucosa.
Conclusions: This report describes a patient with ulcerative colitis, the onset of which coincided with active Epstein-Barr virus and human herpesvirus 6 infection. This is the first demonstration of Epstein-Barr virus in the epithelial cells of the human colon by in situ hybridization and immunohistochemical staining.
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