医学論文


Main bibliograpy







Demonstration of Epstein-Barr Virus Localized in the Colonic and Ileal Mucosa of a Patient with Ulcerative Colitis and review of the literature


Authors: Yoshio Takeda, MD1, Kenzo Takada, MD2, Hitoshi Togashi, MD1, Hiroaki Takeda, MD1, Makoto Sakano, MD3, Yumiko Takeda, MD3, Yukie Osada, MD4, Haruhide Shinzawa, MD1 and Tsuneo Takahashi, MD1

1 The Second Department of Internal Medicine, Yamagata University School of Medicine,
Yamagata 990-9585, Japan.
2 Department of Virology, Cancer Institute, Hokkaido University School of Medicine, Japan.
3 Department of Internal Medicine, Shinoda General Hospital, Japan.
4 Department of Dermatology, Shinoda General Hospital, Japan.

Reprint requests: Yoshio Takeda, M.D. The Second Department of Internal Medicine, Yamagata University School of Medicine, 2-2-2 Iidanishi, Yamagata 990-9585, Japan

Abstract


Background: There has been no report on Epstein-Barr virus localized in the human colonic mucosa.
Methods: In the rectal biopsy specimens from a patient with ulcerative colitis, detection of Epstein-Barr virus and human herpesvirus 6 was performed by the single strand PCR method. To localize Epstein-Barr virus in the paraffin-embedded biopsy specimens from hemorrhagic inflamed rectal mucosa and faintly inflamed terminal ileal mucosa, we attempted to detect Epstein-Barr virus-encoded small RNA-1 by in situ hybridization and Epstein-Barr virus latent membrane protein-1 by immunohistochemical staining.
Results: Epstein-Barr virus and human herpesvirus 6 were detected in the rectal biopsy specimens by the single strand PCR. Positive signals for Epstein-Barr virus-encoded small RNA-1 were recognized in nuclei of some epithelial cells from both the rectum and the terminal ileum. Positive staining for Epstein-Barr virus latent membrane protein-1 was detected in cytoplasm of some epithelial cells and mucosal lymphocytes of the rectal and the terminal ileal mucosa.
Conclusions: This report describes a patient with ulcerative colitis, the onset of which coincided with active Epstein-Barr virus and human herpesvirus 6 infection. This is the first demonstration of Epstein-Barr virus in the epithelial cells of the human colon by in situ hybridization and immunohistochemical staining.







ツールボックス

下から選んでください:

新しいページを作成する
ヘルプ / FAQ もご覧ください。